Melon hybrid SVMF5675 and parents thereof

ABSTRACT

The invention provides seed and plants of melon hybrid SVMF5675 and the parent lines thereof. The invention thus relates to the plants, seeds, and tissue cultures of melon hybrid SVMF5675 and the parent lines thereof and to methods for producing a melon plant produced by crossing such plants with themselves or with another melon plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of melon hybrid SVMF5675 and inbredmelon lines HAR-DV14-4214MO and HAR-DV14-4221AN.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects and pathogens, tolerance to environmental stress,and nutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a melon plant of thehybrid designated SVMF5675, the melon line HAR-DV14-4214MO or melon lineHAR-DV14-4221AN. Also provided are melon plants having all thephysiological and morphological characteristics of such a plant. Partsof these melon plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of melon hybrid SVMF5675and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN comprising anadded heritable trait is provided. The heritable trait may comprise agenetic locus that is, for example, a dominant or recessive allele. Inone embodiment of the invention, a plant of melon hybrid SVMF5675 and/ormelon lines HAR-DV14-4214MO and HAR-DV14-4221AN is defined as comprisinga single locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of melon hybrid SVMF5675 and/ormelon lines HAR-DV14-4214MO and HAR-DV14-4221AN. The melon seed of theinvention may be provided as an essentially homogeneous population ofmelon seed of melon hybrid SVMF5675 and/or melon lines HAR-DV14-4214MOand HAR-DV14-4221AN. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, insome embodiments, seed of hybrid SVMF5675 and/or melon linesHAR-DV14-4214MO and HAR-DV14-4221AN may be defined as forming at leastabout 97% of the total seed, including at least about 98%, 99% or moreof the seed. The seed population may be separately grown to provide anessentially homogeneous population of melon plants designated SVMF5675and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN.

In yet another aspect of the invention, a tissue culture of regenerablecells of a melon plant of hybrid SVMF5675 and/or melon linesHAR-DV14-4214MO and HAR-DV14-4221AN is provided. The tissue culture willpreferably be capable of regenerating melon plants capable of expressingall of the physiological and morphological characteristics of thestarting plant, and of regenerating plants having substantially the samegenotype as the starting plant. Examples of some of the physiologicaland morphological characteristics of the hybrid SVMF5675 and/or melonlines HAR-DV14-4214MO and HAR-DV14-4221AN include those traits set forthin the tables herein. The regenerable cells in such tissue cultures maybe derived, for example, from embryos, meristems, cotyledons, pollen,leaves, anthers, roots, root tips, pistils, flowers, seed and stalks.Still further, the present invention provides melon plants regeneratedfrom a tissue culture of the invention, the plants having all thephysiological and morphological characteristics of hybrid SVMF5675and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN.

In still yet another aspect of the invention, processes are provided forproducing melon seeds, plants and fruit, which processes generallycomprise crossing a first parent melon plant with a second parent melonplant, wherein at least one of the first or second parent melon plantsis a plant of melon line HAR-DV14-4214MO or melon line HAR-DV14-4221AN.These processes may be further exemplified as processes for preparinghybrid melon seed or plants, wherein a first melon plant is crossed witha second melon plant of a different, distinct genotype to provide ahybrid that has, as one of its parents, a plant of melon lineHAR-DV14-4214MO or melon line HAR-DV14-4221AN. In these processes,crossing will result in the production of seed. The seed productionoccurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent melon plant, oftenin proximity so that pollination will occur for example, mediated byinsect vectors. Alternatively, pollen can be transferred manually. Wherethe plant is self-pollinated, pollination may occur without the need fordirect human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent melon plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent melon plants. Yet another step comprisesharvesting the seeds from at least one of the parent melon plants. Theharvested seed can be grown to produce a melon plant or hybrid melonplant.

The present invention also provides the melon seeds and plants producedby a process that comprises crossing a first parent melon plant with asecond parent melon plant, wherein at least one of the first or secondparent melon plants is a plant of melon hybrid SVMF5675 and/or melonlines HAR-DV14-4214MO and HAR-DV14-4221AN. In one embodiment of theinvention, melon seed and plants produced by the process are firstgeneration (F₁) hybrid melon seed and plants produced by crossing aplant in accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridmelon plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid melon plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SVMF5675 and/or melon linesHAR-DV14-4214MO and HAR-DV14-4221AN, the method comprising the steps of:(a) preparing a progeny plant derived from hybrid SVMF5675 and/or melonlines HAR-DV14-4214MO and HAR-DV14-4221AN, wherein said preparingcomprises crossing a plant of the hybrid SVMF5675 and/or melon linesHAR-DV14-4214MO and HAR-DV14-4221AN with a second plant; and (b)crossing the progeny plant with itself or a second plant to produce aseed of a progeny plant of a subsequent generation. In furtherembodiments, the method may additionally comprise: (c) growing a progenyplant of a subsequent generation from said seed of a progeny plant of asubsequent generation and crossing the progeny plant of a subsequentgeneration with itself or a second plant; and repeating the steps for anadditional 3-10 generations to produce a plant derived from hybridSVMF5675 and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN. Theplant derived from hybrid SVMF5675 and/or melon lines HAR-DV14-4214MOand HAR-DV14-4221AN may be an inbred line, and the aforementionedrepeated crossing steps may be defined as comprising sufficientinbreeding to produce the inbred line. In the method, it may bedesirable to select particular plants resulting from step (c) forcontinued crossing according to steps (b) and (c). By selecting plantshaving one or more desirable traits, a plant derived from hybridSVMF5675 and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of melon hybridSVMF5675 and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN, whereinthe plant has been cultivated to maturity, and (b) collecting at leastone melon from the plant.

In still yet another aspect of the invention, the genetic complement ofmelon hybrid SVMF5675 and/or melon lines HAR-DV14-4214MO andHAR-DV14-4221AN is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a melon plant,or a cell or tissue of that plant. A genetic complement thus representsthe genetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make-up of a hybrid cell, tissue orplant. The invention thus provides melon plant cells that have a geneticcomplement in accordance with the melon plant cells disclosed herein,and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SVMF5675 and/or melon lines HAR-DV14-4214MOand HAR-DV14-4221AN could be identified by any of the many well-knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by melon plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a melon plant of the invention with a haploid geneticcomplement of a second melon plant, preferably, another, distinct melonplant. In another aspect, the present invention provides a melon plantregenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features, and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds, and derivatives of melon hybrid SVMF5675, melon lineHAR-DV14-4214MO, and melon line HAR-DV14-4221AN.

Melon hybrid SVMF5675, also known as 15-DV-HAR-5675, is a cantaloupevariety that produces fruit that are round-to-oval in shape andnon-sutured and have yellow skin color at maturity, orange flesh, and along shelf life. The variety comprises resistance to the Cucurbit yellowstunting disorder virus.

A. Origin and Breeding History of Melon Hybrid SVMF5675

The parents of hybrid SVMF5675 are HAR-DV14-4214MO and HAR-DV14-4221AN.The parent lines are uniform and stable, as is a hybrid producedtherefrom. A small percentage of variants can occur within commerciallyacceptable limits for almost any characteristic during the course ofrepeated multiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Melon HybridSVMF5675, Melon Line HAR-DV14-4214MO, and Melon Line HAR-DV14-4221AN

In accordance with one aspect of the present invention, there areprovided plants having the physiological and morphologicalcharacteristics of melon hybrid SVMF5675 and the parent lines thereof.Descriptions of the physiological and morphological characteristics ofsuch plants are presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of Melon HybridSVMF5675 CHARACTERISTIC SVMF5675 Olympic Gold 1. Type common or commonor summer summer 2. Leaf (mature blade of third leaf) shape reniformreniform lobes shallowly lobed shallowly lobed color medium green mediumgreen RHS Color Chart value 137B 137A length (mm) 116.5 129 width (mm)160.7 177.1 surface scabrous scabrous 3. Plant inflorescence, sexexpression monoecious gynoecious (at full flowering) habit vine vine 4.Fruit shape oval oval length (at edible maturity) (cm) 18.3 16.7diameter (at edible 15.9 14.0 maturity) (cm) weight (at edible maturity)(g) 2394.5 1654.7 surface (at edible maturity) netted netted blossomscar (at obscure obscure edible maturity) rib presence (at absent absentedible maturity) shipping quality (at excellent excellent ediblematurity) abscission (at edible maturity) when overripe when ripematurity (number of days from 102 91 seeding to harvest) 5. Flesh colornear cavity (at orange orange edible maturity) RHS Color Chart value 26B26C color in center (at orange orange edible maturity) RHS Color Chartvalue 26B 26C color near rind (at orange orange edible maturity) RHSColor Chart value 26B 26C refractometer % soluable 14.20% 12.90% solids(center of flesh) aroma (at edible maturity) strong strong flavor (atedible maturity) very spicy somewhat spicy 6. Seed Cavity length (mm)115.5 115.1 width (mm) 64.9 62.9 shape in cross section circulartriangular 7. Seed (fully developed and dry seeds after washing anddrying in the shade) number of seeds per fruit 586.9 564.1 grams per1,000 seeds (g) 33 36 8. Rind net presence abundant abundant netdistribution covers entire covers entire fruit fruit coarseness mediumcoarse very coarse interlacing complete complete interstices shallowmedium deep texture hard soft thickness at medial (mm) 1.3 4.0 primarycolor (at yellow green orange edible maturity) RHS Color Chart value154B 26C net color (at edible maturity) gray white orange white RHSColor Chart value 156B 159A primary color (at full maturity) yellowgreen yellow orange RHS Color Chart value N144B 22B net color (at fullmaturity) gray white orange white RHS Color Chart value 195B 159A Theseare typical values. Values may vary due to environment. Values that aresubstantially equivalent are within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of Melon LineHAR-DV14-4214MO CHARACTERISTIC HAR-DV14-4214MO Olympic Gold 1. Typecommon or common or summer summer 2. Leaf (mature blade of third leaf)shape reniform reniform lobes shallowly lobed shallowly lobed color darkgreen medium green RHS Color Chart value 137B 137A length (mm) 130.1 129width (mm) 168.5 177.1 surface glabrous scabrous 3. Plant inflorescence,sex expression monoecious gynoecious (at full flowering) habit vine vine4. Fruit shape oval oval length (at edible 16.9 16.7 maturity) (cm)diameter (at edible 15.2 14.0 maturity) (cm) weight (at edible 1957.31654.7 maturity) (g) surface (at edible maturity) netted netted blossomscar (at obscure obscure edible maturity) rib presence (at absent absentedible maturity) suture surface (at netted netted edible maturity)shipping quality (at excellent excellent edible maturity) abscission (atdo not abscise when ripe edible maturity) maturity (number of days 10191 from seeding to harvest) 5. Flesh color near cavity (at orange orangeedible maturity) RHS Color Chart value 25C 26C color in center (atorange orange edible maturity) RHS Color Chart value 25C 26C color nearrind (at orange orange edible maturity) RHS Color Chart value 25C 26Crefractometer % soluable 13.40% 12.90% solids (center of flesh) aroma(at edible maturity) absent strong flavor (at edible maturity) mildsomewhat spicy 6. Seed Cavity length (mm) 117.6 115.1 width (mm) 67.662.9 shape in cross section circular triangular 7. Seed (fully developedand dry seeds, after washing and drying in the shade) number of seedsper fruit 640.8 564.1 grams per 1,000 seeds (g) 21 36 8. Rind netpresence abundant abundant net distribution covers entire covers entirefruit fruit coarseness fine very coarse interlacing complete completeinterstices shallow medium deep texture hard soft thickness at medial(mm) 1.6 4.0 primary color (at yellow green orange edible maturity) RHSColor Chart value 153C 26C net color (at edible maturity) gray greenorange white RHS Color Chart value 198B 159A primary color (at yellowgreen yellow orange full maturity) RHS Color Chart value 153C 22B netcolor (at full maturity) gray white orange white RHS Color Chart value156C 159A These are typical values. Values may vary due to environment.Values that are substantially equivalent are within the scope of theinvention.

TABLE 3 Physiological and Morphological Characteristics of Melon LineHAR-DV14-4221AN CHARACTERISTIC HAR-DV14-4221AN Olympic Gold 1. Typecommon or common or summer summer 2. Leaf (mature blade of third leaf)shape reniform reniform lobes shallowly lobed shallowly lobed colormedium green medium green RHS Color Chart value 189A 137A length (mm)105.1 129 width (mm) 148.5 177.1 surface scabrous scabrous 3. Plantinflorescence, sex expression gynoecious gynoecious (at full flowering)habit vine vine 4. Fruit length (at edible maturity) 13.1 16.7 (cm)diameter (at edible maturity) 14.2 14.0 (cm) weight (at edible maturity)1482.7 1654.7 (g) shape round oval surface (at edible maturity) nettednetted blossom scar (at edible conspicuous obscure maturity) ribpresence (at edible present absent maturity) suture surface (at ediblenetted netted maturity) shipping quality (at edible fair excellentmaturity) abscission (at edible do not abscise when ripe maturity)maturity (number of days 111 91 from seeding to harvest) 5. Flesh colornear cavity (at orange orange edible maturity) RHS Color Chart value 25C26C color in center (at orange orange edible maturity) RHS Color Chartvalue 25C 26C color near rind (at orange orange edible maturity) RHSColor Chart value 25C 26C refractometer % soluable 15.30% 12.90% solids(center of flesh) aroma (at edible maturity) faint strong flavor (atedible maturity) very spicy somewhat spicy 6. Seed Cavity length (mm)81.3 115.1 width (mm) 60.3 62.9 shape in cross section circulartriangular 7. Seed (fully developed and dry seeds, after washing anddrying in the shade) number of seeds per fruit 325.5 564.1 grams per1,000 seeds (g) 36 36 8. Rind net presence abundant abundant netdistribution covers entire covers entire fruit fruit coarseness mediumcoarse very coarse interlacing complete complete interstices medium deepmedium deep texture hard soft thickness at medial (mm) 3.0 4.0 primarycolor (at yellow green orange edible maturity) RHS Color Chart value145B 26C net color (at edible maturity) gray yellow orange white RHSColor Chart value 160D 159A primary color (at yellow green yellow orangefull maturity) RHS Color Chart value 150C 22B net color (at fullmaturity) gray yellow orange white RHS Color Chart value 160D 159A Theseare typical values. Values may vary due to environment. Values that aresubstantially equivalent are within the scope of the invention.

C. Breeding Melon Plants

One aspect of the current invention concerns methods for producing seedof melon hybrid SVMF5675 involving crossing melon lines HAR-DV14-4214MOand HAR-DV14-4221AN. Alternatively, in other embodiments of theinvention, hybrid SVMF5675, line HAR-DV14-4214MO, or lineHAR-DV14-4221AN may be crossed with itself or with any second plant.Such methods can be used for propagation of hybrid SVMF5675 and/or themelon lines HAR-DV14-4214MO and HAR-DV14-4221AN, or can be used toproduce plants that are derived from hybrid SVMF5675 and/or the melonlines HAR-DV14-4214MO and HAR-DV14-4221AN. Plants derived from hybridSVMF5675 and/or the melon lines HAR-DV14-4214MO and HAR-DV14-4221AN maybe used, in certain embodiments, for the development of new melonvarieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SVMF5675 followed by multiplegenerations of breeding according to such well-known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSVMF5675 and/or melon lines HAR-DV14-4214MO and HAR-DV14-4221AN for thepurpose of developing novel melon lines, it will typically be preferredto choose those plants which either themselves exhibit one or moreselected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, high seed yield, high seedgermination, seedling vigor, high fruit yield, disease tolerance orresistance, adaptability for soil and climate conditions, and delayedfruit ripening. Consumer-driven traits, such as a fruit shape, color,texture, and taste are other examples of traits that may be incorporatedinto new lines of melon plants developed by this invention.

D. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those melon plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalmelon plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental melon plant to whichthe locus or loci from the nonrecurrent parent are transferred is knownas the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a melon plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny melon plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of melon the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiol., 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of melon plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

E. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by moleculargenetic methods. Such methods include, but are not limited to, variousplant transformation techniques and methods for site-specificrecombination, the use of which are well-known in the art, and include,for example, the CRISPR-Cas system, zinc-finger nucleases (ZFNs), andtranscription activator-like effector nucleases (TALENs), among others.

In one embodiment of the invention, genetic transformation may be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well-known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation, anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Nat. Biotechnol., 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Nat. Biotechnol., 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13:344-348, 1994) and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a melon plant according to the invention.Non-limiting examples of particular genes and corresponding phenotypesone may choose to introduce into a melon plant include one or more genesfor insect tolerance, such as a Bacillus thuringiensis (B.t.) gene, pesttolerance such as genes for fungal disease control, herbicide tolerancesuch as genes conferring glyphosate tolerance, and genes for qualityimprovements such as yield, nutritional enhancements, environmental orstress tolerances, or any desirable changes in plant physiology, growth,development, morphology or plant product(s). For example, structuralgenes would include any gene that confers insect tolerance including butnot limited to a Bacillus insect control protein gene as described in WO99/31248, herein incorporated by reference in its entirety, U.S. Pat.No. 5,689,052, herein incorporated by reference in its entirety, U.S.Pat. Nos. 5,500,365 and 5,880,275, herein incorporated by reference intheir entirety. In another embodiment, the structural gene can confertolerance to the herbicide glyphosate as conferred by genes including,but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPSgene (aroA:CP4) as described in U.S. Pat. No. 5,633,435, hereinincorporated by reference in its entirety, or glyphosate oxidoreductasegene (GOX) as described in U.S. Pat. No. 5,463,175, herein incorporatedby reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

F. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-Pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) Color Chart Value: The RHS Color Chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS Color Chart byidentifying the group name, sheet number, and letter, e.g.,Yellow-Orange Group 19A or Red Group 41B.

Self-Pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a melonvariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a melon plant by transformation or sitespecific recombination.

G. Deposit Information

A deposit of melon hybrid SVMF5675 and inbred parent linesHAR-DV14-4214MO and HAR-DV14-4221AN, disclosed above and recited in theclaims, has been made with the American Type Culture Collection (ATCC),10801 University Blvd., Manassas, Va. 20110-2209. The date of depositfor melon hybrid SVMF5675 and inbred parent lines HAR-DV14-4214MO andHAR-DV14-4221AN was Dec. 20, 2017. The accession numbers for thosedeposited seeds of melon hybrid SVMF5675 and inbred parent linesHAR-DV14-4214MO and HAR-DV14-4221AN are ATCC Accession NumberPTA-124686, ATCC Accession Number PTA-124687, and ATCC Accession NumberPTA-124688, respectively. Upon issuance of a patent, all restrictionsupon the deposits will be removed, and the deposits are intended to meetall of the requirements of 37 C.F.R. §§ 1.801-1.809. The deposits willbe maintained in the depository for a period of 30 years, or 5 yearsafter the last request, or for the effective life of the patent,whichever is longer, and will be replaced if necessary during thatperiod.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed:
 1. A melon plant comprising at least a first set of thechromosomes of melon line HAR-DV14-4214MO or melon line HAR-DV14-4221AN,a sample of seed of said lines having been deposited under ATCCAccession Number PTA-124687 and ATCC Accession Number PTA-124688,respectively.
 2. A melon seed that produces the plant of claim
 1. 3. Theplant of claim 1, wherein the plant is a plant of said melon lineHAR-DV14-4214MO or melon line HAR-DV14-4221AN.
 4. The plant of claim 1,wherein the plant is a plant of melon hybrid SVMF5675, a sample of seedof said hybrid having been deposited under ATCC Accession NumberPTA-124686.
 5. The seed of claim 2, wherein the seed is a seed of saidmelon line HAR-DV14-4214MO or melon line HAR-DV14-4221AN.
 6. The seed ofclaim 2, wherein the seed is a seed of melon hybrid SVMF5675, a sampleof seed of said hybrid having been deposited under ATCC Accession NumberPTA-124686.
 7. A plant part of the plant of claim 1, wherein the plantpart comprises a cell of said plant.
 8. A melon plant having all thephysiological and morphological characteristics of the plant of claim 1.9. A tissue culture of regenerable cells of the plant of claim
 1. 10. Amethod of vegetatively propagating the melon plant of claim 1, themethod comprising the steps of: (a) collecting tissue capable of beingpropagated from the plant of claim 1; and (b) propagating a melon plantfrom said tissue.
 11. A method of introducing a trait into a melon line,the method comprising: (a) utilizing as a recurrent parent the plant ofclaim 3 by crossing said plant with a donor melon plant that comprises atrait to produce F₁ progeny; (b) selecting an F₁ progeny that comprisesthe trait; (c) backcrossing the selected F₁ progeny with a plant of thesame melon line used as the recurrent parent in step (a) to producebackcross progeny; (d) selecting a backcross progeny comprising thetrait; and (e) repeating steps (c) and (d) three or more times toproduce a selected fourth or higher backcross progeny that comprises thetrait and otherwise comprises all of the physiological and morphologicalcharacteristics of melon line HAR-DV14-4214MO or melon lineHAR-DV14-4221AN.
 12. A melon plant produced by the method of claim 11.13. A method of producing a melon plant comprising an added trait, themethod comprising introducing a transgene conferring the trait into theplant of claim
 1. 14. A melon plant produced by the method of claim 13,wherein said plant comprises the trait and otherwise comprises all ofthe morphological and physiological characteristics of a melon plantcomprising at least a first set of the chromosomes of melon lineHAR-DV14-4214MO or melon line HAR-DV14-4221AN, a sample of seed of saidlines having been deposited under ATCC Accession Number PTA-124687 andATCC Accession Number PTA-124688, respectively.
 15. A melon plantcomprising at least a first set of the chromosomes of melon lineHAR-DV14-4214MO or melon line HAR-DV14-4221AN, a sample of seed of saidlines having been deposited under ATCC Accession Number PTA-124687 andATCC Accession Number PTA-124688, respectively, further comprising atransgene.
 16. The plant of claim 15, wherein the transgene confers atrait selected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism, and modified protein metabolism.
 17. A melonplant comprising at least a first set of the chromosomes of melon lineHAR-DV14-4214MO or melon line HAR-DV14-4221AN, a sample of seed of saidlines having been deposited under ATCC Accession Number PTA-124687 andATCC Accession Number PTA-124688, respectively, further comprising asingle locus conversion.
 18. The plant of claim 17, wherein the singlelocus conversion confers a trait selected from the group consisting ofmale sterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism, and modified proteinmetabolism.
 19. A method for producing a seed of a melon plant derivedfrom at least one of melon hybrid SVMF5675, melon line HAR-DV14-4214MO,or melon line HAR-DV14-4221AN, the method comprising the steps of: (a)crossing the melon plant of claim 1 with itself or a second melon plant;and (b) allowing seed of a hybrid SVMF5675, line HAR-DV14-4214MO, orline HAR-DV14-4221AN-derived melon plant to form.
 20. A method ofproducing a seed of a hybrid SVMF5675, line HAR-DV14-4214MO, or lineHAR-DV14-4221AN-derived melon plant, the method comprising the steps of:(a) producing a hybrid SVMF5675, line HAR-DV14-4214MO, or lineHAR-DV14-4221AN-derived melon plant from a seed produced by crossing themelon plant of claim 1 with itself or a second melon plant; and (b)crossing the hybrid SVMF5675, line HAR-DV14-4214MO, lineHAR-DV14-4221AN-derived melon plant with itself or a different melonplant to obtain a seed of a further hybrid SVMF5675, lineHAR-DV14-4214MO, or line HAR-DV14-4221AN-derived melon plant.
 21. Themethod of claim 20, the method further comprising repeating saidproducing and crossing steps of (a) and (b) using the seed from saidstep (b) for producing a plant according to step (a) for at least onegeneration to produce a seed of an additional hybrid SVMF5675, lineHAR-DV14-4214MO, or line HAR-DV14-4221AN-derived melon plant.
 22. Amethod of producing a melon fruit, the method comprising: (a) obtainingthe plant of claim 1, wherein the plant has been cultivated to maturity;and (b) collecting a melon fruit from the plant.